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Title | PrimalScheme: primer panels for multiplex |
Description | primalscheme is a tool for designing primer panels for multiplex PCR. It uses a greedy algorithm to find primers for tiling amplicon generation for multiple reference genomes. It works best on viral isolates of known lineages e.g. outbreak |
Keywords | N/A |
WebSite | primalscheme.com |
Host IP | 147.188.173.119 |
Location | United Kingdom |
Site | Rank |
US$1,885,354
Last updated: 2023-05-19 17:45:55
primalscheme.com has Semrush global rank of 5,613,961. primalscheme.com has an estimated worth of US$ 1,885,354, based on its estimated Ads revenue. primalscheme.com receives approximately 217,541 unique visitors each day. Its web server is located in United Kingdom, with IP address 147.188.173.119. According to SiteAdvisor, primalscheme.com is safe to visit. |
Purchase/Sale Value | US$1,885,354 |
Daily Ads Revenue | US$1,741 |
Monthly Ads Revenue | US$52,210 |
Yearly Ads Revenue | US$626,518 |
Daily Unique Visitors | 14,503 |
Note: All traffic and earnings values are estimates. |
Host | Type | TTL | Data |
primalscheme.com. | A | 1798 | IP: 147.188.173.119 |
primalscheme.com. | NS | 1800 | NS Record: dns1.registrar-servers.com. |
primalscheme.com. | NS | 1800 | NS Record: dns2.registrar-servers.com. |
primalscheme.com. | MX | 1800 | MX Record: 20 eforward5.registrar-servers.com. |
primalscheme.com. | MX | 1800 | MX Record: 15 eforward4.registrar-servers.com. |
primalscheme.com. | MX | 1800 | MX Record: 10 eforward1.registrar-servers.com. |
primalscheme.com. | MX | 1800 | MX Record: 10 eforward2.registrar-servers.com. |
primalscheme.com. | MX | 1800 | MX Record: 10 eforward3.registrar-servers.com. |
primalscheme.com. | TXT | 1800 | TXT Record: v=spf1 include:spf.efwd.registrar-servers.com ~all |
Report an issue Protocol GitHub primalscheme primer panels for multiplex PCR Design a new scheme Reset defaults FASTA file… Select a file... Options High-GC mode Pinned Use the standard protocol for these settings. Use the high-GC protocol for these settings. Amplicon size Min/max will be set at 5% either side of target. Scheme name A short name/prefix for your scheme, no spaces. Design scheme View demo inputs Job status: Progress 0% Current Region - Primers considered - Your results Regions 0% Gaps - Coverage - Your scheme assets Getting started Instructions for designing a scheme Download complete genomes from GenBank and concatenate into a single FASTA file. e.g. cat "sequence (1).fasta" "sequence (2).fasta" > input.fasta Align all sequences using Clustal Omega . The genomes should be complete and roughly the same length. Primers are only designed within the limits of the alignment. The first reference will be used for the coordinate system in the output scheme. Check the |
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Domain Name: PRIMALSCHEME.COM Registry Domain ID: 2535186011_DOMAIN_COM-VRSN Registrar WHOIS Server: whois.namecheap.com Registrar URL: http://www.namecheap.com Updated Date: 2020-06-08T10:06:10Z Creation Date: 2020-06-08T10:06:08Z Registry Expiry Date: 2023-06-08T10:06:08Z Registrar: NameCheap, Inc. Registrar IANA ID: 1068 Registrar Abuse Contact Email: abuse@namecheap.com Registrar Abuse Contact Phone: +1.6613102107 Domain Status: clientTransferProhibited https://icann.org/epp#clientTransferProhibited Name Server: DNS1.REGISTRAR-SERVERS.COM Name Server: DNS2.REGISTRAR-SERVERS.COM DNSSEC: unsigned >>> Last update of whois database: 2022-11-04T08:32:45Z <<< |